Course Detail
1. MICROBIOLOGY
I Introduction to Microbiology:
- Historical developments of microbiology, brief account of classification of microorganisms;
- Hackel's three kingdom concept,
- Whittaker's five kingdom concept, three domain concept.
- Prokaryotic and eukaryotic cell,
- Brief account of major groups of microorganisms:
- Bacteria, algae, fungi, protozoa and viruses
II Methods of Microbiology:
- Microscopy: bright field & dark field microscopy,
- fluorescence and immuno-fluorescence microscopy,
- phase contrast and electron (transmission and scanning) microscopy,
- Staining techniques; simple, negative, gram, acid fast, spore and capsule,
- Flagella staining of microorganisms, Isolation and maintenance of microbial cultures from different sources
III Nutrition types and growth of microorganisms:
- Nutritional types (Concept of litho/organo, photo/chemo and auto/ heterotrophs),
- types of media (selective media, differential media, assay and enrichment media), choice of media,
IV Growth of microorganisms:
- Population growth and growth kinetics,
- Methods of growth determination, effect of environmental conditions (pH, temperature, aeration, etc.) continuous culture, diauxic growth, synchronous cultures
V Control of microorganisms:
- Control of microbes by physical (dry and moist heat, pasteurization, tantalization, radiation and filtration) techniques,
- Control of microbes by chemical agents(disinfection sanitization, antisepsis, sterilant and fumigation).
- Determination of phenol coefficient of disinfectant, antibacterial agents,
- Mechanism of resistance to antimicrobial agents, Susceptibility testing of fastidious and anaerobic bacteria
2. PRINCIPLES OF FOOD PROCESSING
I Food processing:
- Introduction to food technology. Food industries/institutions/food scientists of importance in India.
- Properties of foods, food spoilage and shelf-life
II Ambient-Temperature Processing :
- Raw material preparation,
- cooling crops and carcasses,
- cleaning sorting and grading and peeling.
III Low Temperature Processing:
- Chilling and modified atmospheres.
- Theory and its applications
IV Freezing:
- Theory, equipment, effect on foods and micro-organisms.
- Freeze drying and freeze concentration.
V Pressure Processing (Non-thermal technologies-I):
- High-pressure processing; theory, equipment,
- effect on micro-organisms, spores and enzymes
VI Irradiation Processing (Non-thermal technologies-II):
- Effect of radiation in food, use of microwave energy in foods,
- Theory of microwave heating,
- Dielectric properties of food materials,
- Source of radiation,
- Mechanism of preservation,
- Dose determination,
- Working principle of magnetron,
- Microwave blanching
VII Thermal processing 1 :
- Introduction to thermal processing.
- Effect of heat on microorganisms, enzymes and nutritional quality of food.
- Theory and equipment used for heat processing using steam or water (Blanching. Pasteurization, Heat sterilization, Ultra high-temperature (UHT)
VIII Thermal processing -II:
- Drying process, Types of Dryers.
- Dehydration effect in food.
- Technology and effect on food during concentration.
- Post processing technology:
- Edible Barrier Coating, and coating materials.
- Microencapsulation.
IX Packaging theory:
- Packaging theory and types of packaging materials.
- Modified atmospheric packaging (MAP), packaging developments
X Hurdle Technology:
- Concept of hurdle technology.
- Types of Hurdles (physical, physicochemical and microbial).
- Preservation by hurdle technology.
3. FOOD CHEMISTRY AND NUTRITION
I Introduction:
- Food chemistry
- Application and scope
II Carbohydrates:
- Monosaccharide, Disaccharide.
- Oligosaccharides, Polysaccharides
- Classification and properties
III Proteins:
- Sources, amino acids, peptides, protein classification, structure & their denaturation,
- Food proteins
IV Lipids, Oils and Fats:
- Classification and uses of lipids in food,
- Physical and chemical properties,
- Effects of processing on functional properties,
- Sources of oils and fat,
- Classification of lipids,
- Fatty acids (Saturated, Unsaturated, and essential fatty acids)
- ALA, EPA & DHA (omega fatty acids) Physical characteristics of fats.
- Reactions of fats (rancidity and polymerization)